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The morphological statement of nerve fibers was performed by HE, TB staining, and TEM; immunofluorescence of CD34 and VEGF, and immunohistochemical of CD31 were carried out to gauge the neovascularization in regenerated nerve. Triple fluorescence staining of MBP/S100 and NF200/Tuj 1 was employed to judge the regenerated myelin sheath and myelinated axon. Immunofluorescent staining images of C-fos and BDNF have been utilized to judge the calcium signaling pathway of fundamental protein. For a extra detailed means of the experiment, please see Supplementary Note 8. The walking trajectory analysis, gastrocnemius wet-weight ratio, histological evaluation of gastrocnemius, and electrophysiological analysis had been used to measure motor practical restoration. For a extra detailed process of the experiment, please see Supplementary Note 9.
